The outline of Aspergillus oryzae RIB40 (NBRC 100959) proteome
We have analyzed the proteome of Aspergillus oryzae RIB40 (NBRC 100959) grown under liquid culture and membrane-transfer culture by using the following methods.
- (1) Two-dimensional PAGE followed by MALDI-TOFMS
- (2) One-dimensional SDS-PAGE in combination with two-dimensional LC-MS/MS
- (3) Multi-dimensional LC-MS/MS
N-terminal amino acid sequencing by the protein sequencer were also performed.
The result of proteome analysis
Proteome results found the need for addition of new CDSs and modification of registered CDSs. The consortium has been preparing for release of modified data. Currently, the released proteome data is limited to fit to released genetic data.
In recent years, such techniques have been termed proteogenomics.
The results of the proteome analysis are available to be downloaded from the DBRP (Data and Biological Resource Platform).
This study was presented to the following scientific conference.
- 55th ASMS Conference (2007.6)
- “Proteome Analysis of Aspergillus oryzae for Precise Prediction of Protein Coding Regions and Protein Profiling”
- Hanako Ataku, Miyako Mise, Keiko Nishijima, Jun Yamazaki, Kazumi Sasaki, Syuji Yamazaki, Motoaki Sano and Nobuyuki Fujita.
- BMB2007 (2007.12)
- “Proteome Analysis for Precise Prediction of Protein Coding Regions”
- Keiko Nishijima, Jun Yamazaki, Miyako Mise, Hanako Ataku, Kazumi Sasaki, Syuji Yamazaki and Nobuyuki Fujita.
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- Biotechnology Evaluation and Development Division, Biological Resource Center, National Institute of Technology and Evaluation
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