The outline of Tetragenococcus halophilus NBRC 12172 proteome

日本語で表示

We have analyzed the proteome of halotolerant lactic acid bacterium, Tetragenococcus halophilus NBRC 12172, cultured with added salt concentration of 0% and 10% by using the following methods.

(1) Soluble fraction: Two-dimensional PAGE followed by MALDI-TOFMS
(2) Soluble fraction: One-dimensional SDS-PAGE in combination with two-dimensional LC-MS/MS
(3) Soluble fraction: Whole shotgun proteomics
(4) Membrane enriched fraction (treated by lysozyme and ultracentrifuged): One-dimensional SDS-PAGE in combination with two-dimensional LC-MS/MS
(5) Membrane enriched fraction (disrupted on dry ice and ultracentrifuged): on-column digestion followed by LC/MS/MS
(6) Membrane enriched fraction (disrupted on dry ice): on-column digestion followed by LC/MS/MS

N-terminal amino acid sequencing by the protein sequencer were also performed.

The result of proteome analysis

Tetragenococcus halophilus NBRC 12172 cultured under 0% salt condition was in more stressed condition rather than in 10% condition. It indicated that this strain acquires energy using high salt condition environment. In addition, it was suggested that the salt resistance was related to uptake of osmoregulation substance and membrane glycoprotein.

The results of the proteome analysis are available to be downloaded from the DBRP (Data and Biological Resource Platform).

This study was presented to the following scientific conference.

Keiko Nishijima, Jun Yamazaki, Miyako Mise, Hanako Ataku, Takashi Yabuki, Asao Ichige, Yasuji Koyama, Kazumi Sasaki, Syuji Yamazaki, Nobuyuki Fujita

“Proteome analysis of halotolerant lactic acid bacterium, Tetragenococcus halophilus: Effect of NaCl concentrations in media.”
Annual Meeting of Japan Society for Bioscience, Biotechnology, and Agrochemistry, 3P0973A (2009)

Takashi Yabuki, Keiko Nishijima, Miyako Mise, Hanako Ataku, Asao Ichige, Syuji Yamazaki, Kazumi Sasaki, Nobuyuki Fujita, Yasuji Koyama

“Proteome analysis of halotolerant lactic acid bacterium, Tetragenococcus halophilus: Improvement of preparation method for membrane protein fraction.”
Annual Meeting of Japan Society for Bioscience, Biotechnology, and Agrochemistry, 3P0974B (2009)

Tetragenococcus halophilus NBRC 12172 proteome data in DBRP

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