The outline of Brevibacillus brevis 47 proteome

We have analyzed the proteome of Brevibacillus brevis 47 (NBRC 100599) under the following conditions.

1. Shotgun proteomics (SDS-PAGE in combination with LC-MS/MS)

2. Peptide Mass Fingerprinting (two-dimensional PAGE followed by MALDI-TOFMS)

3. Multi-dimensional LC-MS/MS

4. N-terminal amino acid sequencing by the protein sequencer

The result of proteome analysis

A large number of proteins of B. brevis 47 (35% of predicted ORFs) were identified by comprehensive proteome analysis.
5 proteins matched the genomic regions not previously assigned as ORFs.
The annotation of the B. brevis 47 genomic sequence was refined and 5,950 ORFs were finally predicted. In recent years, such techniques have been termed proteogenomics.
Genes for queuosine biosynthesis were most abundantly expressed during logarithmic growth phase.
Magnesium ions seemed to enhance the expression of two catabolic pathways of B. brevis 47.

All results of the proteome analysis are available to be downloaded from the DBRP (Data and Biological Resource Platform).

This study was presented to the following scientific conference.

54th ASMS Conference (2006.5): “Comprehensive proteome analysis of Brevibacillus brevis 47”; Hanako Ataku, Miyako Mise, Keiko Nishijima, Rie Otsuka, Jun Yamazaki, Syuji Yamazaki, Kazumi Sasaki, Shinichi Tago and Nobuyuki Fujita
BMB2007 (2007.12): “Proteome Analysis for Precise Prediction of Protein Coding Regions”; Keiko Nishijima, Jun Yamazaki, Miyako Mise, Hanako Ataku, Kazumi Sasaki, Syuji Yamazaki and Nobuyuki Fujita

Biotechnology Evaluation and Development Division, Biological Resource Center, National Institute of Technology and Evaluation: Address:2-49-10 Nishihara, Shibuya-ku, Tokyo 1510066, Japan MAP
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