The outline of Rhodococcus opacus B4 (NBRC 108011) proteome
We have analyzed the proteome of Rhodococcus opacus B4 (NBRC 108011) under the following four conditions.
- (1) Original strain (tolerance to organic solvent)
- (2) Reduced-tolerance strain
- (3) sigB deficient strain (deleted-tolerance)
- (4) Original strain cultured with added oleyl alcohol
These samples were analyzed by Peptide Mass Fingerprinting (two-dimensional PAGE followed by MALDI-TOFMS) and shotgun proteomics (one-dimensional SDS-PAGE in combination with two-dimensional LC-MS/MS).
N-terminal amino acid sequencing by the protein sequencer were also performed.
The result of proteome analysis
A total of 3164 proteins were identified.
All results of the proteome analysis are available to be downloaded from the DBRP (Data and Biological Resource Platform).
This study was presented to the following scientific conference.
- Jun Fukuda, Keiko Nishijima, Miyako Mise, Hanako Ataku, Kazumi Sasaki, Syuji Yamazaki, Kohsuke Honda, Junichi Kato, Nobuyuki Fujita, Hisao Ohtake
- “Proteome Analysis of Organic Solvent-tolerant Bacterium Rhodococcus opacus strain B4”
- Annual Meeting of the Japan Society for Environmental Biotechnology, P-32 (2009)
This study was sponsored by New Energy and Industrial Technology Development Organization (NEDO).
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