The outline of Aspergillus fumigatus NBRC 113945 proteome
We cultured Aspergillus fumigatus NBRC 113945 isolated from a heat exchanger of air-conditioner at 37°C and analyzed the comprehensive proteome of the samples (1) and (2) shown below. The extracted proteins were separated by SDS-PAGE, digested in gel by trypsin and analyzed by the high-performance liquid chromatograph mass spectrometer (LC/MS/MS).
- (1) Intracellular (Cells disrupted in liquid nitrogen and extracted by acetone precipitation)
- (2) Extracellular (Extraction from broth by acetone precipitation)
The result of proteome analysis
A total of 1527 proteins were detected from intracellular samples and 83 proteins were detected from extracellular samples. Among these, 44 proteins were reported to be allergens.
The results of the proteome analysis are available to be downloaded from the DBRP (Data and Biological Resource Platform).
The number of detected proteins
Analytical Instrument
The mass spectrometer used for the analysis is introduced below.
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